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FOXA1 antibody [FOXA1/1519]

Anti-FOXA1 antibody [FOXA1/1519] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34740
Anti-FOXA1 antibody [FOXA1/1519] used in IHC (Paraffin sections) (IHC-P). GTX34740
Anti-FOXA1 antibody [FOXA1/1519] used in Western Blot (WB). GTX34740
Anti-FOXA1 antibody [FOXA1/1519] used in Flow cytometry (FACS). GTX34740
Anti-FOXA1 antibody [FOXA1/1519] used in IHC (Paraffin sections) (IHC-P). GTX34740

Cat. No. GTX34740

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 FOXA1/1519

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat
Package
100 μg ($529)
See all FOXA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 0.5-1μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human FOXA1 protein (aa372-472) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FOXA1 , HNF3A , TCF3A , forkhead box A1

Cellular Localization

Nucleus

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FOXA1 antibody [FOXA1/1519] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34740

GTX34740 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34740 FOXA1 antibody [FOXA1/1519].
Green : Primary antibody
Red : Actin

Anti-FOXA1 antibody [FOXA1/1519] used in IHC (Paraffin sections) (IHC-P). GTX34740

GTX34740 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34740 FOXA1 antibody [FOXA1/1519].

Anti-FOXA1 antibody [FOXA1/1519] used in Western Blot (WB). GTX34740

GTX34740 WB Image

WB analysis of HeLa cell lysate using GTX34740 FOXA1 antibody [FOXA1/1519].

Anti-FOXA1 antibody [FOXA1/1519] used in Flow cytometry (FACS). GTX34740

GTX34740 FACS Image

FACS analysis of PFA-fixed MCF-7 cells using GTX34740 FOXA1 antibody [FOXA1/1519].
Blue : Primary antibody
Red : Isotype control

Anti-FOXA1 antibody [FOXA1/1519] used in IHC (Paraffin sections) (IHC-P). GTX34740

GTX34740 IHC-P Image

IHC-P analysis of rat colon tissue using GTX34740 FOXA1 antibody [FOXA1/1519].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529