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FOXA1 antibody

Orthogonal
Anti-FOXA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100308
Anti-FOXA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100308
Anti-FOXA1 antibody used in ChIP assay (ChIP assay). GTX100308
Anti-FOXA1 antibody used in Immunoprecipitation (IP). GTX100308
Anti-FOXA1 antibody used in Western Blot (WB). GTX100308
Anti-FOXA1 antibody used in Western Blot (WB). GTX100308
Anti-FOXA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100308

Cat. No. GTX100308

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay, ChIP-seq

Reactivity

 Monopterus albus, Human
Package
100 μl ($399),
25 μl ($169)
See all FOXA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240823

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

forkhead box A1 , HNF3A , TCF3A

Cellular Localization

Nucleus

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FOXA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100308

GTX100308 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1(GTX100308) antibody at 1:1000 dilution.

Anti-FOXA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100308

GTX100308 IHC-P Image

FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FOXA1 antibody used in ChIP assay (ChIP assay). GTX100308

GTX100308 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11651-6.

Anti-FOXA1 antibody used in Immunoprecipitation (IP). GTX100308

GTX100308 IP Image

FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 μg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 μg of FOXA1 antibody (GTX100308) 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody (GTX100308) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-FOXA1 antibody used in Western Blot (WB). GTX100308

GTX100308 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody (GTX100308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-FOXA1 antibody used in Western Blot (WB). GTX100308

GTX100308 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody (GTX100308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-FOXA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100308

GTX100308 IHC-P Image

FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Roza Berhanu Lemma et al. Epigenetics Chromatin 2022; 15 (1) : 13 Pioneer transcription factors are associated with the modulation of DNA methylation patterns across cancers.
Application : WB, IP
Reactivity : Human
Dylane Detilleux et al. Epigenetics Chromatin 2022; 15 (1) : 10 Pan-cancer predictions of transcription factors mediating aberrant DNA methylation.
Application : WB, ChIP-seq
Xin Wang et al. Cell Biosci 2020; 10 (1) : 129 DNA methylation modification is associated with gonadal differentiation in Monopterus albus.
Application : ChIP assay
Reactivity : Monopterus albus
Lin ZS et al. Am J Cancer Res 2018; 8 (10) : 1989-2004 FOXA1 transcriptionally up-regulates cyclin B1 expression to enhance chondrosarcoma progression.
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169