GeneTex
United States (US)

FOXA1 antibody

GTX100308_39435_IHC_19011506_286.jpg
GTX100308_39435_IFA_19011506_325.jpg
GTX100308_WB_19011506_811.jpg
GTX100308_39435_IHC_2_19011506_153.jpg
GTX100308_39435_ChIP_19011506_876.jpg
GTX100308_39435_IP_19011506_746.jpg

Cat No. GTX100308

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HNF3A antibody, MGC33105 antibody, TCF3A antibody, FOXA1 antibody, HNF-3-alpha antibody, HNF-3A antibody, hepatocyte nuclear factor 3-alpha antibody, "hepatocyte nuclear factor 3, alpha antibody", transcription factor 3A antibody, forkhead box protein A1 antibody, TCF-3A antibody, forkhead box A1 antibody

Cellular Localization

Nucleus

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100308_39435_IHC_19011506_286.jpg

GTX100308 IHC-P Image

FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100308_39435_IFA_19011506_325.jpg

GTX100308 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1(GTX100308) antibody at 1:1000 dilution.

GTX100308_WB_19011506_811.jpg

GTX100308 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX100308 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100308_39435_IHC_2_19011506_153.jpg

GTX100308 IHC-P Image

FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
FOXA1 antibody (GTX100308) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100308_39435_ChIP_19011506_876.jpg

GTX100308 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11651-6.

GTX100308_39435_IP_19011506_746.jpg

GTX100308 IP Image

FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 μg whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 μg of FOXA1 antibody (GTX100308) 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody (GTX100308) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169