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FOXA2 antibody [3C10]

Anti-FOXA2 antibody [3C10] used in IHC (Paraffin sections) (IHC-P). GTX84485
Anti-FOXA2 antibody [3C10] used in IHC (Paraffin sections) (IHC-P). GTX84485
Anti-FOXA2 antibody [3C10] used in Flow cytometry (FACS). GTX84485
Anti-FOXA2 antibody [3C10] used in Western Blot (WB). GTX84485
Anti-FOXA2 antibody [3C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84485
Anti-FOXA2 antibody [3C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84485
Anti-FOXA2 antibody [3C10] used in Western Blot (WB). GTX84485

Cat No. GTX84485

Host

Mouse

Clonality

Monoclonal

Clone Name

3C10

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50-1:100
IHC-P 1:50
FACS 1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

forkhead box A2 , HNF3B , TCF3B

Cellular Localization

Nucleus,Cytoplasm

Background

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs | FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis | regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Database

Research Area

DATA IMAGES

Anti-FOXA2 antibody [3C10] used in IHC (Paraffin sections) (IHC-P). GTX84485

GTX84485 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84485 FOXA2 antibody [3C10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FOXA2 antibody [3C10] used in IHC (Paraffin sections) (IHC-P). GTX84485

GTX84485 IHC-P Image

IHC-P analysis of lung tissue using GTX84485 FOXA2 antibody [3C10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FOXA2 antibody [3C10] used in Flow cytometry (FACS). GTX84485

GTX84485 FACS Image

FACS analysis of HEK293T cells transfected with either FOXA2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84485 FOXA2 antibody [3C10].
Dilution : 1:1000

Anti-FOXA2 antibody [3C10] used in Western Blot (WB). GTX84485

GTX84485 WB Image

WB analysis of HEK293T cells transfected with FOXA2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84485 FOXA2 antibody [3C10].
Loading : 5 ug per lane

Anti-FOXA2 antibody [3C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84485

GTX84485 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with FOXA2 plasmid using GTX84485 FOXA2 antibody [3C10].

Anti-FOXA2 antibody [3C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84485

GTX84485 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84485 FOXA2 antibody [3C10].
Dilution : 1:50

Anti-FOXA2 antibody [3C10] used in Western Blot (WB). GTX84485

GTX84485 WB Image

WB analysis of A549 cell lysate using GTX84485 FOXA2 antibody [3C10].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

There are currently no references for FOXA2 antibody [3C10] (GTX84485). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FOXA2 antibody [3C10] (GTX84485). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf