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FOXA2 antibody, C-term

Anti-FOXA2 antibody, C-term used in Western Blot (WB). GTX25074
Anti-FOXA2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25074
Anti-FOXA2 antibody, C-term used in Western Blot (WB). GTX25074

Cat. No. GTX25074

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all FOXA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 4-8μg/ml

Note :

IHC-P
Human Prostate shows nuclear staining in the secretory cells of the gland.
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX25074-PEP).

Product Note

This antibody is expected to recognise both reported isoforms: NP_068556.2; NP_710141.1

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GVYSRPIMNSS, from the C Terminus of the protein sequence according to NP_068556.2; NP_710141.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box A2 , HNF3B , TCF3B

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-FOXA2 antibody, C-term used in Western Blot (WB). GTX25074

GTX25074 WB Image

WB analysis of human liver lysate using GTX25074 FOXA2 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-FOXA2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25074

GTX25074 IHC-P Image

IHC-P analysis of human prostate using GTX25074 FOXA2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-FOXA2 antibody, C-term used in Western Blot (WB). GTX25074

GTX25074 WB Image

WB analysis of rat lung lysate using GTX25074 FOXA2 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399