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FOXC1 antibody, C-term

Anti-FOXC1 antibody, C-term used in Western Blot (WB). GTX25079
Anti-FOXC1 antibody, C-term used in Flow cytometry (FACS). GTX25079
Anti-FOXC1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25079
Anti-FOXC1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25079

Cat. No. GTX25079

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($399)
See all FOXC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P Assay dependent
FACS 10μg/ml
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX25079-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RTSGAFVYDCSKF, from the C Terminus of the protein sequence according to NP_001444.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_368058

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box C1 , ARA , ASGD3 , FKHL7 , FREAC-3 , FREAC3 , IGDA , IHG1 , IRID1 , RIEG3

Cellular Localization

Nucleus

Background

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FOXC1 antibody, C-term used in Western Blot (WB). GTX25079

GTX25079 WB Image

WB analysis of HEK293 (A) MCF7 nuclear (B) and Negative control Daudi (C) cell lysate using GTX25079 FOXC1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-FOXC1 antibody, C-term used in Flow cytometry (FACS). GTX25079

GTX25079 FACS Image

FACS analysis of PFA fixed HEK293 cells using GTX25079 FOXC1 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-FOXC1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25079

GTX25079 IHC-P Image

IHC-P analysis of human cerebellum using GTX25079 FOXC1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-FOXC1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25079

GTX25079 IHC-P Image

IHC-P analysis of human spleen using GTX25079 FOXC1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

Application Reference

Yue Y et al. J Biol Chem 2018; 293 (2) : 638-650 The transcription factor Foxc1a in zebrafish directly regulates expression of nkx2.5, encoding a transcriptional regulator of cardiac progenitor cells.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399