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FOXC2 antibody, C-term

Anti-FOXC2 antibody, C-term used in Flow cytometry (FACS). GTX25060
Anti-FOXC2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25060
Anti-FOXC2 antibody, C-term used in Western Blot (WB). GTX25060

Cat. No. GTX25060

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($399)
See all FOXC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 2-4μg/ml
FACS 10μg/ml

Note :

IHC-P
Human Breast Cancer shows nuclear staining with some weak cytoplasm staining.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX25060-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RHAAPYSYDCTKY, from the C Terminus of the protein sequence according to NP_005242.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box C2 , FKHL14 , LD , MFH-1 , MFH1

Cellular Localization

Nucleus

Background

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FOXC2 antibody, C-term used in Flow cytometry (FACS). GTX25060

GTX25060 FACS Image

FACS analysis of PFA fixed HEK293 cells using GTX25060 FOXC2 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 1μg/ml

Anti-FOXC2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX25060

GTX25060 IHC-P Image

IHC-P analysis of human breast cancer using GTX25060 FOXC2 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer Ph 9
Dilution : 2μg/ml

Anti-FOXC2 antibody, C-term used in Western Blot (WB). GTX25060

GTX25060 WB Image

WB analysis of nuclear HEK293 (A) and negative control human Pancreas (B) lysate (RIPA buffer, 35μg total protein per lane) using GTX25060 FOXC2 antibody, C-term.
Dilution : 2μg/ml (nuclear HEK293) and 1μg/ml (human pancreas)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399