GeneTex
United States (US)

FOXD3 antibody

FOXD3-antibody-GTX87777-IHC-1_18122102_301.jpg
FOXD3-antibody-GTX87777-WB-1_18122102_405.jpg

Cat No. GTX87777

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

47 kDa. ( Note )

Specificity/Sensitivity

FOXD3 Antibody detects endogenous levels of total FOXD3 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human FOXD3.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Forkhead Box D3,Ais1,Genesis,Hfh2,Vamas2,Foxd3

Background

Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.

Database

Research Area

DATA IMAGES
FOXD3-antibody-GTX87777-IHC-1_18122102_301.jpg

GTX87777 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using FOXD3 Antibody. The picture on the right is treated with the synthesized peptide.

FOXD3-antibody-GTX87777-WB-1_18122102_405.jpg

GTX87777 WB Image

Western blot analysis of extracts from HuvEc/293 cells, using FOXD3 Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 349