GeneTex
United States (US)

FOXE1 antibody

GTX100275_39435_IP_19011506_820.jpg
GTX100275_WB_19011506_514.jpg

Cat No. GTX100275

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HeLaS3
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Forkhead Box E1,Fkhl15,Foxe2,Hfkh4,Hfkl5,Nmtc4,Titf2,Ttf-2,Ttf2,Foxe1

Cellular Localization

Nucleus

Background

This intronless gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene functions as a thyroid transcription factor which likely plays a crucial role in thyroid morphogenesis. Mutations in this gene are associated with congenital hypothyroidism and cleft palate with thyroid dysgenesis. The map localization of this gene suggests it may also be a candidate gene for squamous cell epithelioma and hereditary sensory neuropathy type I. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100275_39435_IP_19011506_820.jpg

GTX100275 IP Image

FOXE1 antibody immunoprecipitates FOXE1 protein in IP experiments.
IP Sample: HeLa whole cell lysate/extract
A. 40 μg HeLa whole cell lysate/extract
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of FOXE1 protein by 2 μg of FOXE1 antibody (GTX100275)
15% SDS-PAGE
The immunoprecipitated FOXE1 protein was detected by FOXE1 antibody (GTX100275) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100275_WB_19011506_514.jpg

GTX100275 WB Image

Sample (30 ug of whole cell lysate)
A: HeLaS3
10% SDS PAGE
GTX100275 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169