APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , HCT116 , FOXI1-transfected 293T , HepG2 nuclear extract
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human FOXI1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10622904
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
forkhead box I1 , FKH10 , FKHL10 , FREAC-6 , FREAC6 , HFH-3 , HFH3
Background
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408](/templates/default/js/lightbox/images/loading.gif "Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408")
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GTX114408 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using FOXI1(GTX114408) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX114408 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXI1(GTX114408) antibody at 1:200 dilution.
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GTX114408 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114408 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114408 WB Image
HepG2 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114408 IHC-P Image
FOXI1 antibody [N2C3] detects FOXI1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX114408 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for FOXI1 antibody [N2C3] (GTX114408). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FOXI1 antibody [N2C3] (GTX114408). Be the first to share your experience with this product.
![Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408](/upload/website/prouct_img/small/GTX114408/GTX114408_40184_IHC_w_23060501_783.webp "Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114408](/upload/website/prouct_img/small/GTX114408/GTX114408_40184_IFA_w_23060501_748.webp "Anti-FOXI1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/small/GTX114408/GTX114408_40184_20171201_WB_2_w_23060501_758.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/small/GTX114408/GTX114408_40184_20180615_WB_B_w_23060501_579.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/small/GTX114408/GTX114408_40184_20180706_WB_Fraction_w_23060501_282.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408](/upload/website/prouct_img/small/GTX114408/GTX114408_40184_20150601_IHC_M_w_23060501_374.webp "Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/small/GTX114408/GTX114408_43628_20190705_WB_22072519_174.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408](/upload/website/prouct_img/normal/GTX114408/GTX114408_40184_IHC_w_23060501_783.webp "Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114408](/upload/website/prouct_img/normal/GTX114408/GTX114408_40184_IFA_w_23060501_748.webp "Anti-FOXI1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/normal/GTX114408/GTX114408_40184_20171201_WB_2_w_23060501_758.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/normal/GTX114408/GTX114408_40184_20180615_WB_B_w_23060501_579.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/normal/GTX114408/GTX114408_40184_20180706_WB_Fraction_w_23060501_282.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408](/upload/website/prouct_img/normal/GTX114408/GTX114408_40184_20150601_IHC_M_w_23060501_374.webp "Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408")
![Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408](/upload/website/prouct_img/normal/GTX114408/GTX114408_43628_20190705_WB_22072519_174.webp "Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408")