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FOXI1 antibody [N2C3]

Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408
Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408
Anti-FOXI1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114408
Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408
Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408
Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408
Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408

Cat No. GTX114408

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , FOXI1-transfected 293T , HepG2 nuclear extract

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FOXI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622904

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

forkhead box I1 , FKH10 , FKHL10 , FREAC-6 , FREAC6 , HFH-3 , HFH3

Background

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408

GTX114408 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408

GTX114408 WB Image

HepG2 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXI1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114408

GTX114408 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXI1(GTX114408) antibody at 1:200 dilution.

Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408

GTX114408 IHC-P Image

FOXI1 antibody [N2C3] detects FOXI1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408

GTX114408 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXI1 antibody [N2C3] used in Western Blot (WB). GTX114408

GTX114408 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (GTX114408) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXI1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114408

GTX114408 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using FOXI1(GTX114408) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for FOXI1 antibody [N2C3] (GTX114408). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FOXI1 antibody [N2C3] (GTX114408). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169