GeneTex
United States (US)

FOXM1 antibody

KO/KD
GTX102170_43103_20181026_WB_KO_watermark_19011519_173.jpg
GTX102170_43103_20180202_WB_19011506_323.jpg
GTX102170_43103_20180119_WB_B_19011506_585.jpg

Cat No. GTX102170

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , FOXM1-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.32mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FOXM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FKHL16 antibody, FOXM1B antibody, HFH-11 antibody, HFH11 antibody, HNF-3 antibody, INS-1 antibody, MPHOSPH2 antibody, MPP-2 antibody, MPP2 antibody, PIG29 antibody, TGT3 antibody, TRIDENT antibody, FOXM1 antibody, hepatocyte nuclear factor 3 forkhead homolog 11 antibody, Forkhead, drosophila, homolog-like 16 antibody, forkhead-related protein FKHL16 antibody, HNF-3/fork-head homolog 11 antibody, M-phase phosphoprotein 2 antibody, transcription factor Trident antibody, forkhead box protein M1 antibody, winged-helix factor from INS-1 cells antibody, MPM-2 reactive phosphoprotein 2 antibody, forkhead box M1 antibody

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
GTX102170_43103_20181026_WB_KO_watermark_19011519_173.jpg

GTX102170 WB Image

KO/KD

Wild-type (WT) and FOXM1 knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX102170_43103_20180202_WB_19011506_323.jpg

GTX102170 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102170_43103_20180119_WB_B_19011506_585.jpg

GTX102170 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf