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FOXM1 antibody

KO/KD Orthogonal Overexpression
Anti-FOXM1 antibody used in Western Blot (WB). GTX102170
Anti-FOXM1 antibody used in Western Blot (WB). GTX102170
Anti-FOXM1 antibody used in Western Blot (WB). GTX102170
Anti-FOXM1 antibody used in Western Blot (WB). GTX102170
Anti-FOXM1 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX102170
Anti-FOXM1 antibody used in Western Blot (WB). GTX102170
Anti-FOXM1 antibody used in Western Blot (WB). GTX102170
Anti-FOXM1 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX102170
Anti-FOXM1 antibody used in Immunoprecipitation (IP). GTX102170
Anti-FOXM1 antibody used in Western Blot (WB). GTX102170
Anti-FOXM1 antibody used in ChIP assay (ChIP assay). GTX102170

Cat. No. GTX102170

Reference 15

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FOXM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

FOXM1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FOXM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box M1 , FKHL16 , FOXM1A , FOXM1B , FOXM1C , HFH-11 , HFH11 , HNF-3 , INS-1 , MPHOSPH2 , MPP-2 , MPP2 , PIG29 , TRIDENT

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-FOXM1 antibody used in Western Blot (WB). GTX102170

GTX102170 WB Image

KO/KD

Wild-type (WT) and FOXM1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-FOXM1 antibody used in Western Blot (WB). GTX102170

GTX102170 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXM1 antibody used in Western Blot (WB). GTX102170

GTX102170 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FOXM1 antibody used in Western Blot (WB). GTX102170

GTX102170 WB Image

KO/KD

The data was published in the 2015 in Oncotarget. PMID: 25938542

Anti-FOXM1 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX102170

GTX102170 WB IP Image

Orthogonal

The data was published in the journal Sci Rep in 2016. PMID: 27845446

Anti-FOXM1 antibody used in Western Blot (WB). GTX102170

GTX102170 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2016. PMID: 27845446

Anti-FOXM1 antibody used in Western Blot (WB). GTX102170

GTX102170 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2016. PMID: 27845446

Anti-FOXM1 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX102170

GTX102170 WB IP Image

The data was published in the journal Sci Rep in 2016. PMID: 27845446

Anti-FOXM1 antibody used in Immunoprecipitation (IP). GTX102170

GTX102170 IP Image

The data was published in the journal Genome Biol in 2013.PMID: 23347430

Anti-FOXM1 antibody used in Western Blot (WB). GTX102170

GTX102170 WB Image

The data was published in the journal Genome Biol in 2013.PMID: 23347430

Anti-FOXM1 antibody used in ChIP assay (ChIP assay). GTX102170

GTX102170 ChIP assay Image

The data was published in the journal Genome Biol in 2015. PMID: 26100407

REFERENCE

Application Reference

Kim MY et al. Am J Cancer Res 2021; 11 (6) : 2944-2959 Niclosamide exerts anticancer effects through inhibition of the FOXM1-mediated DNA damage response in prostate cancer.
Application : WB
Reactivity : Human
Corvaisier M et al. Commun Biol 2021; 4 (1) : 767 The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells.
Application : ChIP assay
Shufeng Li et al. Onco Targets Ther 2020; 13 : 12383-12395 CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.
Application : WB
Reactivity : Human
Lim SH et al. Pharmaceuticals (Basel) 2020; 13 (10)
Application : WB
Reactivity : Human
Zeng WJ et al. J Cell Mol Med 2020; Aberrant ASPM expression mediated by transcriptional regulation of FoxM1 promotes the progression of gliomas.
Application : WB, IHC-P
Reactivity : Human
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REVIEW

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Application Reference
Kim MY et al. Am J Cancer Res 2021; 11 (6):2944-2959 Niclosamide exerts anticancer effects through inhibition of the FOXM1-mediated DNA damage response in prostate cancer.
Application : WB
Reactivity : Human
Corvaisier M et al. Commun Biol 2021; 4 (1):767 The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells.
Application : ChIP assay
Shufeng Li et al. Onco Targets Ther 2020; 13:12383-12395 CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.
Application : WB
Reactivity : Human
Lim SH et al. Pharmaceuticals (Basel) 2020; 13 (10)
Application : WB
Reactivity : Human
Zeng WJ et al. J Cell Mol Med 2020; Aberrant ASPM expression mediated by transcriptional regulation of FoxM1 promotes the progression of gliomas.
Application : WB, IHC-P
Reactivity : Human
Kyuno T et al. Pancreas 2020; 49 (2):273-280 Glucose-Dependent FOXM1 Promotes Epithelial-to-Mesenchymal Transition Via Cellular Metabolism and Targeting Snail in Human Pancreatic Cancer.
Application : WB
Reactivity : Human
Manzione MG et al. Mol Biol Cell 2020;:mbcE19120698 Co-regulation of the antagonistic RepoMan:Aurora-B pair in proliferating cells.
Ziegler Y et al. NPJ Breast Cancer 2019; 5:45 Suppression of FOXM1 activities and breast cancer growth in vitro and in vivo by a new class of compounds.
Huang C et al. Mol Ther Oncolytics 2019; 14:196-212
Application : ChIP assay
Reactivity : Human
Zhang T et al. J Cancer 2019; 10 (15):3501-3516 KIAA0101 is a novel transcriptional target of FoxM1 and is involved in the regulation of hepatocellular carcinoma microvascular invasion by regulating epithelial-mesenchymal transition.
Application : WB, IHC-P, ChIP assay
Reactivity : Human
Cohn O et al. Sci Rep 2016; 6:37115 Chromatin associated SETD3 negatively regulates VEGF expression.
Application : WB, IP
Reactivity : Human
Xie Q et al. Oncotarget 2015; 6 (15):13241-54 CDC20 maintains tumor initiating cells.
Sanders DA et al. Genome Biol 2015; 16 (Epub):130 FOXM1 binds directly to non-consensus sequences in the human genome.
Application : ChIP assay
Reactivity : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
Application : WB
Reactivity : Human
Sanders DA et al. Genome Biol 2013; 14 (1):R6 Genome-wide mapping of FOXM1 binding reveals co-binding with estrogen receptor alpha in breast cancer cells.
Application : ChIP assay
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169