APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , mouse thymus , rat thymus
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXN1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240834
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
forkhead box N1 , FKHL20 , RONU , WHN
Cellular Localization
Nucleus
Background
Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX104849 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104849 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104849 WB Image
FOXN1 antibody [C3], C-term detects FOXN1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500.
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GTX104849 WB Image
FOXN1 antibody [C3], C-term detects FOXN1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXN1 antibody [C3], C-term (GTX104849) diluted at 1:500.
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REFERENCE
There are currently no references for FOXN1 antibody [C3], C-term (GTX104849). Be the first to share your publications with this product.
REVIEW
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