Phosphate-buffered saline, pH 7.4, containing no preservatives
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Amino acids 242-655 of FKHR expressed in E. coli.
Protein G purified
From tissue culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Forkhead Box O1 , Fkh1 , Fkhr , Foxo1A , Foxo1
FKHR-7H3 recognizes FKHR (ForKHead in Rhabdomyosarcoma), a 70a protein, which is a member of the forkhead homeotic gene family of transcription factors (FKH/HNF-3). Members of the forkhead family can be classified and grouped by their degree of homology within the 100-120aa region that is necessary and sufficient for DNA binding and binding-site specificity. The FKHR subfamily of forkhead proteins is relatively divergent from the other members of the forkhead transcription factor family. With all members in this subfamily containing a signature insertion of 5 amino acids next to the helix 3 in the middle of the forkhead domain, and lack the well conserved amino terminal KPPY sequence found in most forkhead genes. Recently, it has been shown that members of the FKHR gene family are phosphorylated by Akt and this phosphorylation regulates its cellular localization promoting cell survival.