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FOXO1 antibody

Anti-FOXO1 antibody used in Western Blot (WB). GTX110724

Cat. No. GTX110724

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Squirrel, Xenopus
Package
100 μl ($399),
25 μl ($169)
See all FOXO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FOXO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617375

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box O1 , FKH1 , FKHR , FOXO1A

Cellular Localization

Cytoplasm , Nucleus

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FOXO1 antibody used in Western Blot (WB). GTX110724

GTX110724 WB Image

FOXO1 antibody detects FOXO1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
7.5 % SDS-PAGE
FOXO1 antibody (GTX110724) dilution: 1:500

REFERENCE

Application Reference

Chang TC et al. Pharmacol Res 2021; 163 : 105298 Ugonin J improves metabolic disorder and ameliorates nonalcoholic fatty liver disease by regulating the AMPK/AKT signaling pathway.
Application : WB
Reactivity : Human
Azorsa DO et al. Mod Pathol 2020; (Epub) Immunohistochemical detection of PAX-FOXO1 fusion proteins in alveolar rhabdomyosarcoma using breakpoint specific monoclonal antibodies.
Application : WB
Reactivity : Human
Luu BE et al. Cell Stress Chaperones 2020; (Epub) The regulation of Akt and FoxO transcription factors during dehydration in the African clawed frog (Xenopus laevis).
Application : WB
Reactivity : Xenopus
Zhang Y et al. PeerJ 2016; 4 : e2317 Transcriptional activation of muscle atrophy promotes cardiac muscle remodeling during mammalian hibernation.
Application : WB
Reactivity : Squirrel
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169