GeneTex
United States (US)

FOXO1 (phospho Ser319) antibody

GTX32183_20190131_WB_19013101_151.jpg
GTX32183_20190131_IHC-P_19013101_584.jpg
GTX32183_20190131_ICCIF_19013101_361.jpg

Cat No. GTX32183

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of FOXO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Forkhead Box O1,Fkh1,Fkhr,Foxo1A,Foxo1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32183_20190131_WB_19013101_151.jpg

GTX32183 WB Image

WB analysis of MCF7 (A), HeLa (B), NIH3T3 (C) whole cell lysates using GTX32183 FOXO1 (phospho Ser319) antibody.

GTX32183_20190131_IHC-P_19013101_584.jpg

GTX32183 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32183 FOXO1 (phospho Ser319) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32183_20190131_ICCIF_19013101_361.jpg

GTX32183 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32183 FOXO1 (phospho Ser319) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Package List Price ($)
$ 319