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FOXO1 (phospho Ser319) antibody

Anti-FOXO1 (phospho Ser319) antibody used in IHC (Paraffin sections) (IHC-P). GTX32183
Anti-FOXO1 (phospho Ser319) antibody used in Western Blot (WB). GTX32183
Anti-FOXO1 (phospho Ser319) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32183

Cat. No. GTX32183

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FOXO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of FOXO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box O1 , FKH1 , FKHR , FOXO1A

Cellular Localization

Cytoplasm,Nucleus

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FOXO1 (phospho Ser319) antibody used in IHC (Paraffin sections) (IHC-P). GTX32183

GTX32183 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32183 FOXO1 (phospho Ser319) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-FOXO1 (phospho Ser319) antibody used in Western Blot (WB). GTX32183

GTX32183 WB Image

WB analysis of MCF7 (A), HeLa (B), NIH3T3 (C) whole cell lysates using GTX32183 FOXO1 (phospho Ser319) antibody.

Anti-FOXO1 (phospho Ser319) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32183

GTX32183 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32183 FOXO1 (phospho Ser319) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399