ICC/IF analysis of formalin-fixed HeLa cells using GTX32183 FOXO1 (phospho Ser319) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

WB analysis of MCF7 (A), HeLa (B), NIH3T3 (C) whole cell lysates using GTX32183 FOXO1 (phospho Ser319) antibody.

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32183 FOXO1 (phospho Ser319) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)