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FOXO1 (phospho Ser319) antibody

Anti-FOXO1 (phospho Ser319) antibody used in Western Blot (WB). GTX50196
Anti-FOXO1 (phospho Ser319) antibody used in IHC (Paraffin sections) (IHC-P). GTX50196
Anti-FOXO1 (phospho Ser319) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50196

Cat. No. GTX50196

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FOXO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 319 (T-S-S(p)-N-A) derived from human FOXO1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box O1 , FKH1 , FKHR , FOXO1A

Cellular Localization

Cytoplasm,Nucleus

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FOXO1 (phospho Ser319) antibody used in Western Blot (WB). GTX50196

GTX50196 WB Image

WB analysis of extracts from 3T3 cells untreated or treated with serum starvation using GTX50196 FOXO1 (phospho Ser319) antibody.

Anti-FOXO1 (phospho Ser319) antibody used in IHC (Paraffin sections) (IHC-P). GTX50196

GTX50196 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50196 FOXO1 (phospho Ser319) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-FOXO1 (phospho Ser319) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50196

GTX50196 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50196 FOXO1 (phospho Ser319) antibody.

REFERENCE

Application Reference

Chi JN et al. Theranostics 2022; 12 (13) : 5744-5760 MAP4K3/GLK inhibits Treg differentiation by direct phosphorylating IKKβ and inducing IKKβ-mediated FoxO1 nuclear export and Foxp3 downregulation.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399