APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-1μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX17026-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GAKQASSQSWVPG, from the C Terminus of the protein sequence according to NP_001446.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
forkhead box O3 , AF6q21 , FKHRL1 , FKHRL1P2 , FOXO2 , FOXO3A
Cellular Localization
Cytoplasm, cytosol , Nucleus , Mitochondrion matrix , Mitochondrion outer membrane
Background
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX17026 IHC-P Image
IHC-P analysis of human uterus using GTX17026 FOXO3A antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX17026 WB Image
WB analysis of human heart lysate with (B) and without (A) blocking with the immunising peptide using GTX17026 FOXO3A antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX17026 IHC-P Image
IHC-P analysis of human tonsil using GTX17026 FOXO3A antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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REFERENCE
There are currently no references for FOXO3A antibody, C-term (GTX17026). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FOXO3A antibody, C-term (GTX17026). Be the first to share your experience with this product.
