GeneTex
United States (US)

FOXO3A antibody [C3], C-term

GTX100277_39443_20160303_WB_M_shRNA_watermark_19011506_806.jpg
GTX100277_39443_WB_M_Brain_19011506_867.jpg
GTX100277_39443_WB_19011506_862.jpg
GTX100277_40268_IHC_19011506_410.jpg
GTX100277_40268_WB_19011506_149.jpg
GTX100277_39443_IP_19011506_678.jpg
GTX100277_39443_20160113_IFA_19011506_627.jpg

Cat No. GTX100277

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Squirrel
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , Mouse brain , C2C12 , Jurkat , Raji

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXO3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Forkhead Box O3,Af6Q21,Fkhrl1,Fkhrl1P2,Foxo2,Foxo3A,Foxo3

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100277_39443_20160303_WB_M_shRNA_watermark_19011506_806.jpg

GTX100277 WB Image

Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100277_39443_WB_M_Brain_19011506_867.jpg

GTX100277 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX100277 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100277_39443_WB_19011506_862.jpg

GTX100277 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX100277 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100277_40268_IHC_19011506_410.jpg

GTX100277 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A(GTX100277) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100277_40268_WB_19011506_149.jpg

GTX100277 WB Image

Sample (30 µg of whole cell lysate)
A: Jurkat
B: Raji
7.5% SDS PAGE
GTX100277 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100277_39443_IP_19011506_678.jpg

GTX100277 IP Image

FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 μg of FOXO3A antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100277_39443_20160113_IFA_19011506_627.jpg

GTX100277 ICC/IF Image

FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FOXO3A protein stained by FOXO3A antibody [C3], C-term (GTX100277) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169