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FOXO3A antibody

Anti-FOXO3A antibody used in Western Blot (WB). GTX82877
Anti-FOXO3A antibody used in Western Blot (WB). GTX82877
Anti-FOXO3A antibody used in Western Blot (WB). GTX82877
Anti-FOXO3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82877

Cat. No. GTX82877

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all FOXO3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF 1-2 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues L N(654) V G N F T G A K Q A S S Q S W V(670) of human FOXO3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box O3 , Fkhrl1 , Foxo3a

Background

FoxO (forkhead box O) proteins are a family of transcription factors characterized by the distinct forkhead domain. The FoxO proteins act in the insulin signaling pathway, but FoxO3 has also been shown to play a role in the regulation of apoptosis, cell cycle progression and cancer. Several splice variants of FoxO3 have been reported.

Database

Research Area

DATA IMAGES

Anti-FOXO3A antibody used in Western Blot (WB). GTX82877

GTX82877 WB Image

WB analysis of 25 ug of PC-3 (lane 1), Jurkat (lane 2), rat heart (lane 3) and mouse brain (lane 4) using GTX82877 FOXO3A antibody.
Dilution : 1:1000

Anti-FOXO3A antibody used in Western Blot (WB). GTX82877

GTX82877 WB Image

WB analysis of rat lung lysate using GTX82877 FOXO3A antibody.

Anti-FOXO3A antibody used in Western Blot (WB). GTX82877

GTX82877 WB Image

WB analysis of whole cell extracts (20 μg lysate) of Jurkat (Lane 1), KARPAS-299 (Lane 2), MCF7 (Lane 3), COLO 205 (lane 4) and HT-29 (lane 5) using GTX82877 FOXO3A antibody.
Dilution : 1:500-1:2000

Anti-FOXO3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82877

GTX82877 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX82877 FOXO3A antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399