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FOXP3 antibody [3G3] (APC)

Anti-FOXP3 antibody [3G3] (APC) used in Flow cytometry (FACS). GTX80283-07

Cat. No. GTX80283-07

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G3

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Mouse, Rhesus Monkey
Package
100 μg ($359)
See all FOXP3 products
PRODUCT

Summary

The 3G3 antibody reacts with mouse Foxp3, a 50-55 kDa transcription factor which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is expressed at constitutively high levels in Treg cells, which are further identified as being CD4+ CD25+. In resting conventional T cells (CD4+ CD25-) Foxp3 expression is restricted, and upon TCR activation is expressed only transiently and in a small proportion of cells. However, the growth factor TGF-beta has been shown to induce expression of Foxp3 in naïve T cells, driving their development into Foxp3+ Tregs, which are called “induced” or “adaptive” Tregs. These cells are phenotypically similar to so-called “natural” Tregs (CD4+ CD25high Foxp3+) which originate in the thymus and comprise the majority of Treg cells. Tregs are critical for maintaining peripheral tolerance and are implicated in the development of autoimmunity.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

This antibody recognizes N-terminal region of FoxP3.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Full-length His-tagged recombinant murine FoxP3

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box P3 , JM2 , scurfin , sf

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-FOXP3 antibody [3G3] (APC) used in Flow cytometry (FACS). GTX80283-07

GTX80283-07 FACS Image

FACS analysis of C57Bl/6 splenocytes stained FITC anti-mouse CD4 antibody, followed by intracellular staining with GTX80283-07 FOXP3 antibody [3G3] (APC).
Right panel : Co-stained with FITC anti-mouse CD4 antibody and GTX80283-07
Left panel : Co-stained with FITC anti-mouse CD4 antibody and APC mouse IgG1 isotype control
Antibody amount : 0.125 μg

REFERENCE

Clone Reference

Marc A Gavin et al. Proc Natl Acad Sci U S A . 2006; 103 (17):6659-64 Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development
Application : FACS
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 359