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FOXP3 antibody, C-term

Anti-FOXP3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89752

Cat. No. GTX89752

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Mouse
Package
100 μg ($399)
See all FOXP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 2-4μg/ml

Note :

IHC-P
Mouse Thymus shows nuclear staining in some patches of thymocytes.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89752-PEP).

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide with sequence KRSQRPNKCSNP, from the C Terminus of the protein sequence according to NP_473380.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box P3 , JM2 , scurfin , sf

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-FOXP3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89752

GTX89752 IHC-P Image

IHC-P analysis of mouse thymus (Right panel shows staining without primary antibody as negative control) using GTX89752 FOXP3 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml

REFERENCE

Application Reference

Herrera FG et al. Cancer Discov 2022; 12 (1) : 108-133 Low-Dose Radiotherapy Reverses Tumor Immune Desertification and Resistance to Immunotherapy.
Application : IHC-P
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399