WB analysis of human muscle (A), Molt-4 (B) and negative control pancreas (C) lysate using GTX89934 FOXP3 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

ICC/IF analysis of CD25-sorted (Treg) human blood cells using GTX89934 FOXP3 antibody, C-term.
A: Primary antibody and FITC conjigated secondary antibody, B: Phase contrast.

FACS analysis of PFA fixed Jurkat cells using GTX89934 FOXP3 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

ICC/IF analysis of PFA fixed U2OS cells using GTX89934 FOXP3 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

IHC-P analysis of human tonsil using GTX89934 FOXP3 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 1μg/ml
A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.