GeneTex
United States (US)

FOXP3 antibody, C-term

GTX89934_67_ICC-IF_18122102_748.jpg
GTX89934_31_FACS_18122102_289.jpg
GTX89934_68_ICC-IF_18122102_156.jpg
GTX89934_1195_WB_18122102_544.jpg
GTX89934_207_IHC-P_18122102_916.jpg

Cat No. GTX89934

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF 2-4μg/ml
IHC-P Assay dependent
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the nucleoplasm of U2OS cells.

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89934-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2; NP_001107849.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AIID Antibody , PIDX Antibody , IPEX Antibody , DIETER Antibody , JM2 Antibody , XPID Antibody

Background

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX89934_67_ICC-IF_18122102_748.jpg

GTX89934 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX89934 FOXP3 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX89934_31_FACS_18122102_289.jpg

GTX89934 FACS Image

FACS analysis of PFA fixed Jurkat cells using GTX89934 FOXP3 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10µg/ml

GTX89934_68_ICC-IF_18122102_156.jpg

GTX89934 ICC/IF Image

ICC/IF analysis of CD25-sorted (Treg) human blood cells using GTX89934 FOXP3 antibody, C-term.
A: Primary antibody and FITC conjigated secondary antibody, B: Phase contrast.

GTX89934_1195_WB_18122102_544.jpg

GTX89934 WB Image

WB analysis of human muscle (A), Molt-4 (B) and negative control pancreas (C) lysate using GTX89934 FOXP3 antibody, C-term.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

GTX89934_207_IHC-P_18122102_916.jpg

GTX89934 IHC-P Image

IHC-P analysis of human tonsil using GTX89934 FOXP3 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 1µg/ml
A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.

Package List Price ($)
$ 329