APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 2-4μg/ml |
| Assay dependent |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the nucleoplasm of U2OS cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89934-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2; NP_001107849.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
forkhead box P3 , AIID , DIETER , IPEX , JM2 , PIDX , XPID
Cellular Localization
Nucleus , Cytoplasm
Background
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89934 IHC-P Image
IHC-P analysis of human tonsil using GTX89934 FOXP3 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 1μg/ml
A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.
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GTX89934 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX89934 FOXP3 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89934 FACS Image
FACS analysis of PFA fixed Jurkat cells using GTX89934 FOXP3 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX89934 ICC/IF Image
ICC/IF analysis of CD25-sorted (Treg) human blood cells using GTX89934 FOXP3 antibody, C-term.
A: Primary antibody and FITC conjigated secondary antibody, B: Phase contrast.
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GTX89934 WB Image
WB analysis of human muscle (A), Molt-4 (B) and negative control pancreas (C) lysate using GTX89934 FOXP3 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for FOXP3 antibody, C-term (GTX89934). Be the first to share your publications with this product.
REVIEW
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