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FOXP3 antibody [C3], C-term

Anti-FOXP3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107737
Anti-FOXP3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107737
Anti-FOXP3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107737
Anti-FOXP3 antibody [C3], C-term used in Western Blot (WB). GTX107737

Cat No. GTX107737

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

mouse brain
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse FOXP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Forkhead Box P3,Jm2,Scurfin,Sf,Foxp3

Cellular Localization

Nucleus

Background

Probable transcription factor. Plays a critical role in the control of immune response.

Database

DATA IMAGES
Anti-FOXP3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107737

GTX107737 IHC-P Image

FOXP3 antibody [C3], C-term detects FOXP3 protein at cytoplasm and nucleus in mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FOXP3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107737

GTX107737 IP Image

FOXP3 antibody [C3], C-term immunoprecipitates FOXP3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of FOXP3 protein by 4 μg FOXP3 antibody [C3], C-term (GTX107737)
10 % SDS-PAGE
The immunoprecipitated FOXP3 protein was detected by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-FOXP3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107737

GTX107737 ICC/IF Image

FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-FOXP3 antibody [C3], C-term used in Western Blot (WB). GTX107737

GTX107737 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX107737 diluted at 1:1000

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169