GeneTex
United States (US)

FOXP3 antibody [C3], C-term

GTX107737_39715_20170929_IHC-P_M_19011507_290.jpg
GTX107737_39715_IP_19011507_748.jpg
GTX107737_39715_20160303_IFA_19011507_153.jpg
GTX107737_39715_WB_M_Brain_19011507_199.jpg

Cat No. GTX107737

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Rat, Sheep, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse FOXP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

scurfy antibody, forkhead box protein P3 antibody, JM2 antibody, scurfin antibody, sf antibody, Foxp3 antibody, forkhead box P3 antibody

Cellular Localization

Nucleus

Background

Probable transcription factor. Plays a critical role in the control of immune response.

Database

DATA IMAGES
GTX107737_39715_20170929_IHC-P_M_19011507_290.jpg

GTX107737 IHC-P Image

FOXP3 antibody [C3], C-term detects FOXP3 protein at cytoplasm and nucleus in mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107737_39715_IP_19011507_748.jpg

GTX107737 IP Image

FOXP3 antibody [C3], C-term immunoprecipitates FOXP3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of FOXP3 protein by 4 μg FOXP3 antibody [C3], C-term (GTX107737)
10 % SDS-PAGE
The immunoprecipitated FOXP3 protein was detected by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX107737_39715_20160303_IFA_19011507_153.jpg

GTX107737 ICC/IF Image

FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX107737_39715_WB_M_Brain_19011507_199.jpg

GTX107737 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX107737 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169