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FOXP3 antibody [MF23] (APC)

Anti-FOXP3 antibody [MF23] (APC) used in Flow cytometry (FACS). GTX01488-07

Cat. No. GTX01488-07

Reference 6

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MF23

Isotype

 IgG2b

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($319)
See all FOXP3 products
PRODUCT

Summary

The MF23 antibody reacts with mouse Foxp3, a 50-55 kDa transcription factor which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is a member of the forkhead box or winged helix family of transcription factors and is expressed at constitutively high levels in Treg cells. Forced expression of Foxp3 in conventional T cells results in the upregulation of Treg associated molecules such as CD25, CTLA-4, and GITR and is sufficient to impart suppressive functional activity to these cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent

Note :

FACS
Recommend using the Transcription Factor Staining Buffer Kit for optimal staining results with this antibody.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-FOXP3 antibody [MF23] (APC) used in Flow cytometry (FACS). GTX01488-07

GTX01488-07 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01488-07 FOXP3 antibody [MF23] (APC).
Right panel : primary antibody
Left panel : isotype control
antibody amount : 0.25 μg (5 μl)

REFERENCE

Clone Reference

2012; CD25+ T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses.
2012; Repression of cyclic adenosine monophosphate upregulation disarms and expands human regulatory T cells.
2011; Blocking of α4β7 gut-homing integrin during acute infection leads to decreased plasma and gastrointestinal tissue viral loads in simian immunodeficiency virus-infected rhesus macaques.
2008; Epigenetic inheritance of DNA methylation limits activation-induced expression of FOXP3 in conventional human CD25-CD4+ T cells.
2007; Effective proliferation of human regulatory T cells requires a strong costimulatory CD28 signal that cannot be substituted by IL-2.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319