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FOXP3 antibody

Orthogonal
Anti-FOXP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04725
Anti-FOXP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04725
Anti-FOXP3 antibody used in Western Blot (WB). GTX04725
Anti-FOXP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04725
Anti-FOXP3 antibody used in Flow cytometry (FACS). GTX04725

Cat. No. GTX04725

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($419)
See all FOXP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3 μg/mL
ICC/IF 10 μg/ml
IHC-P 6-8 μg/mL
FACS 10 μg/mL
ELISA 1:8000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline, 0.5% BSA

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2 and NP_001107849.1.

Purification


From serum

Purity

Purified by ammonium sulphate precipitation followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AIID,DIETER,FOXP3,IPEX,JM2,PIDX,XPID,forkhead box P3

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FOXP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04725

GTX04725 IHC-P Image

IHC-P analysis of human spleen tissue using GTX04725 FOXP3 antibody.
Left : Primary antibody
Right : No primary antibody
Antigen retrieval : Heat mediated citrate buffer (pH 6.0)
Dilution : 8 μg/mL

Anti-FOXP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04725

GTX04725 ICC/IF Image

ICC/IF analysis of PFA-fixed U2OS cells using GTX04725 FOXP3 antibody.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10 μg/mL

Anti-FOXP3 antibody used in Western Blot (WB). GTX04725

GTX04725 WB Image

Orthogonal

WB analysis of various samples using GTX04725 FOXP3 antibody.
Lane A : Human muscle tissue lysate
Lane B : Molt-4 whole cell lysate
Lane C : Human pancreas tissue lysate (negative control)
Dilution : 1 μg/mL

Anti-FOXP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04725

GTX04725 ICC/IF Image

ICC/IF analysis of CD25-sorted (Treg) human blood cells using GTX04725 FOXP3 antibody.
A : Detected by FITC
B : in phase contrast

Anti-FOXP3 antibody used in Flow cytometry (FACS). GTX04725

GTX04725 FACS Image

FACS analysis of PFA-fixed Jurkat cells using GTX04725 FOXP3 antibody.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 419