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FOXP3 antibody

Overexpression
Anti-FOXP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX30696
Anti-FOXP3 antibody used in Immunohistochemistry (IHC). GTX30696
Anti-FOXP3 antibody used in Western Blot (WB). GTX30696

Cat. No. GTX30696

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse
Package
100 μl ($439)
See all FOXP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
IHC-P 1:200 - 1:1000
IHC-Fr 1:50
IHC 1:50 - 1:1000

Note :

WB
The use of GTX30696 for mouse is based on IHC testing. For WB using mouse samples, we recommend GTX107737.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431). [UniProt# Q9BZS1]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box P3 , AIID , DIETER , IPEX , JM2 , PIDX , XPID

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FOXP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX30696

GTX30696 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX30696 FOXP3 antibody.
Dilution : 1:100

Anti-FOXP3 antibody used in Immunohistochemistry (IHC). GTX30696

GTX30696 IHC Image

IHC analysis of mouse thymus tissue using GTX30696 FOXP3 antibody and CD4 antibody(Double stains) or CD4 antibody only(Controls).

Anti-FOXP3 antibody used in Western Blot (WB). GTX30696

GTX30696 WB Image

Overexpression

WB analysis of multiple samples using GTX30696 FOXP3 antibody.
Lane 1 : Human CD4+/CD25+ PBL cell
Lane 2 : 293 overexpressing human FOXP3 <
Lane 3 : 293 overexpressing mouse FOXP3
Lane 4 : 293 mock

REFERENCE

Application Reference

Zhang Z et al. Clin Transl Med 2022; 12 (11) : e1072 Characterization of the tumour microenvironment phenotypes in malignant tissues and pleural effusion from advanced osteoblastic osteosarcoma patients.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 439