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FRK antibody [5F3]

Anti-FRK antibody [5F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84484
Anti-FRK antibody [5F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84484
Anti-FRK antibody [5F3] used in Flow cytometry (FACS). GTX84484
Anti-FRK antibody [5F3] used in Flow cytometry (FACS). GTX84484
Anti-FRK antibody [5F3] used in Western Blot (WB). GTX84484

Cat. No. GTX84484

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F3

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Dog
Package
100 μl ($519)
See all FRK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human FRK (NP_002022) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fyn related Src family tyrosine kinase , GTK , PTK5 , RAK

Cellular Localization

Cytoplasm,Nucleus

Background

Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor.

Database

Research Area

DATA IMAGES

Anti-FRK antibody [5F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84484

GTX84484 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84484 FRK antibody [5F3].

Anti-FRK antibody [5F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84484

GTX84484 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with FRK plasmid using GTX84484 FRK antibody [5F3].

Anti-FRK antibody [5F3] used in Flow cytometry (FACS). GTX84484

GTX84484 FACS Image

FACS analysis of Jurkat cells using GTX84484 FRK antibody [5F3].
Red : Primary antibody
Blue : Negative control antibody

Anti-FRK antibody [5F3] used in Flow cytometry (FACS). GTX84484

GTX84484 FACS Image

FACS analysis of HeLa cells using GTX84484 FRK antibody [5F3].
Red : Primary antibody
Blue : Negative control antibody

Anti-FRK antibody [5F3] used in Western Blot (WB). GTX84484

GTX84484 WB Image

WB analysis of various cell lines using GTX84484 FRK antibody [5F3].
Loading : 35 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519