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FRK antibody [6D2]

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481
Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481
Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481
Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481
Anti-FRK antibody [6D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84481
Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481
Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481
Anti-FRK antibody [6D2] used in Western Blot (WB). GTX84481
Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481
Anti-FRK antibody [6D2] used in Flow cytometry (FACS). GTX84481

Cat. No. GTX84481

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all FRK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human FRK (NP_002022) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fyn related Src family tyrosine kinase , GTK , PTK5 , RAK

Cellular Localization

Cytoplasm,Nucleus

Background

Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor.

Database

Research Area

DATA IMAGES

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481

GTX84481 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84481 FRK antibody [6D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481

GTX84481 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84481 FRK antibody [6D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481

GTX84481 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84481 FRK antibody [6D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481

GTX84481 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84481 FRK antibody [6D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FRK antibody [6D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84481

GTX84481 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with FRK plasmid using GTX84481 FRK antibody [6D2].

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481

GTX84481 IHC-P Image

IHC-P analysis of human colon tissue using GTX84481 FRK antibody [6D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481

GTX84481 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84481 FRK antibody [6D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FRK antibody [6D2] used in Western Blot (WB). GTX84481

GTX84481 WB Image

WB analysis of HEK293T cells transfected with FRK plasmid (Right) or empty vector (Left) for 48 hrs using GTX84481 FRK antibody [6D2].
Loading : 5 ug per lane

Anti-FRK antibody [6D2] used in IHC (Paraffin sections) (IHC-P). GTX84481

GTX84481 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84481 FRK antibody [6D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FRK antibody [6D2] used in Flow cytometry (FACS). GTX84481

GTX84481 FACS Image

FACS analysis of HEK293T cells transfected with either FRK plasmid(Red) or empty vector control plasmid(Blue) using GTX84481 FRK antibody [6D2].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519