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FRS2 antibody

Anti-FRS2 antibody used in Western Blot (WB). GTX103288
Anti-FRS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103288
Anti-FRS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103288

Cat No. GTX103288

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all FRS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Rat, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FRS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibroblast growth factor receptor substrate 2 , FRS1A , FRS2A , FRS2alpha , SNT , SNT-1 , SNT1

Cellular Localization

Endomembrane system

Background

Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.

Database

Research Area

DATA IMAGES

Anti-FRS2 antibody used in Western Blot (WB). GTX103288

GTX103288 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody (GTX103288) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FRS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103288

GTX103288 IHC-P Image

FRS2 antibody detects FRS2 protein at membrane on HSC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HSC-3 xenograft.
FRS2 antibody (GTX103288) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FRS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103288

GTX103288 IHC-P Image

FRS2 antibody detects FRS2 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FRS2 antibody (GTX103288) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Zhang K et al. Cancer Res 2013; 73 (4) : 1298-307 Amplification of FRS2 and activation of FGFR/FRS2 signaling pathway in high-grade liposarcoma.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169