GeneTex
United States (US)

FRS2 antibody

GTX103288_39918_20170615_WB_19011506_163.jpg
GTX103288_39918_IHC_19011506_359.jpg
GTX103288_39918_20150206_IHC_M_19011506_432.jpg

Cat No. GTX103288

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
See all FRS2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Rat, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FRS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibroblast Growth Factor Receptor Substrate 2,Frs1A,Frs2A,Frs2Alpha,Snt,Snt-1,Snt1,Frs2

Cellular Localization

Endomembrane system

Background

Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.

Database

Research Area

DATA IMAGES
GTX103288_39918_20170615_WB_19011506_163.jpg

GTX103288 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with FRS2 antibody (GTX103288) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103288_39918_IHC_19011506_359.jpg

GTX103288 IHC-P Image

FRS2 antibody detects FRS2 protein at membrane on HSC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HSC-3 xenograft.
FRS2 antibody (GTX103288) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX103288_39918_20150206_IHC_M_19011506_432.jpg

GTX103288 IHC-P Image

FRS2 antibody detects FRS2 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FRS2 antibody (GTX103288) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Zhang K et al. Cancer Res 2013; 73 (4) : 1298-307 Amplification of FRS2 and activation of FGFR/FRS2 signaling pathway in high-grade liposarcoma.
Application : WB
Submit a Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169