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FSD1 antibody

Anti-FSD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131769
Anti-FSD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131769
Anti-FSD1 antibody used in Western Blot (WB). GTX131769

Cat. No. GTX131769

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FSD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1200
IHC Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FSD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2782962

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibronectin type III and SPRY domain containing 1 , GLFND , MIR1

Cellular Localization

Centrosome , Nucleus , Cytoplasm

Background

This gene encodes a centrosome associated protein that is characterized by an N-terminal coiled-coil region downstream of B-box (BBC) domain, a central fibronectin type III domain, and a C-terminal repeats in splA and RyR (SPRY) domain. The encoded protein associates with a subset of microtubules and may be involved in the stability and organization of microtubules during cytokinesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FSD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131769

GTX131769 IHC-P Image

FSD1 antibody detects FSD1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FSD1 antibody (GTX131769) diluted at 1:1200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FSD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131769

GTX131769 ICC/IF Image

FSD1 antibody detects FSD1 protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FSD1 protein stained by FSD1 antibody (GTX131769) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FSD1 antibody used in Western Blot (WB). GTX131769

GTX131769 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FSD1 antibody (GTX131769) diluted at 1:1000.

REFERENCE

Application Reference

Sohni A et al. Cell Rep 2019; 26 (6) : 1501-1517.e4 The Neonatal and Adult Human Testis Defined at the Single-Cell Level.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169