U87-MG whole cell extract and conditioned medium (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FSTL1 antibody [C2C3], C-term (GTX106425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using FSTL1(GTX106425) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using FSTL1(GTX106425) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX106425 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.