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FTO antibody, N-term

Anti-FTO antibody, N-term used in Flow cytometry (FACS). GTX81611
Anti-FTO antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81611
Anti-FTO antibody, N-term used in Western Blot (WB). GTX81611

Cat. No. GTX81611

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all FTO products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FTO.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FTO alpha-ketoglutarate dependent dioxygenase , ALKBH9 , BMIQ14 , GDFD

Cellular Localization

Nucleus,Nucleus speckle,Cytoplasm

Background

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-FTO antibody, N-term used in Flow cytometry (FACS). GTX81611

GTX81611 FACS Image

FACS analysis of NCI-H292 cells using GTX81611 FTO antibody, N-term.
Top histogram : negative control
Bottom histogram : NCI-H292 cells

Anti-FTO antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81611

GTX81611 IHC-P Image

IHC-P analysis of human brain tissue using GTX81611 FTO antibody, N-term.

Anti-FTO antibody, N-term used in Western Blot (WB). GTX81611

GTX81611 WB Image

WB analysis of NCI-H460 cell lysate (35ug/lane) using GTX81611 FTO antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399