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FUBP1 antibody

Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104579
Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579
Anti-FUBP1 antibody used in Western Blot (WB). GTX104579
Anti-FUBP1 antibody used in Western Blot (WB). GTX104579
Anti-FUBP1 antibody used in Immunoprecipitation (IP). GTX104579
Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579
Anti-FUBP1 antibody used in ChIP assay (ChIP assay). GTX104579

Cat No. GTX104579

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

far upstream element binding protein 1 , FBP , FUBP , hDH V

Cellular Localization

Nucleus

Background

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq]

Database

DATA IMAGES
Anti-FUBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104579

GTX104579 ICC/IF Image

FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (GTX104579) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579

GTX104579 IHC-P Image

FUBP1 antibody detects FUBP1 protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FUBP1 antibody (GTX104579) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FUBP1 antibody used in Western Blot (WB). GTX104579

GTX104579 WB Image

Hela whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUBP1 antibody used in Western Blot (WB). GTX104579

GTX104579 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX104579) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUBP1 antibody used in Immunoprecipitation (IP). GTX104579

GTX104579 IP Image

FUBP-1 antibody immunoprecipitates FUBP-1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 μg of FUBP-1 antibody (GTX104579) 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody (GTX1104579) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-FUBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104579

GTX104579 IHC-P Image

FUBP1 antibody detects FUBP1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
FUBP1 antibody (GTX104579) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FUBP1 antibody used in ChIP assay (ChIP assay). GTX104579

GTX104579 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.
ChIP experiment and primer designs are based on EMBO Rep. 2011 Sep 1;12(9):924-30.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169