GeneTex
United States (US)

FUBP1 antibody

Orthogonal
GTX115154_40401_IFA_18121410_420.jpg
GTX115154_40401_20150410_IFA_18121410_830.jpg
GTX115154_40401_IHC_18121410_178.jpg
GTX115154_40401_20150410_IFA_2_18121410_965.jpg
GTX115154_40401_20170420_WB_M_R_18121410_143.jpg
GTX115154_40401_20170420_WB_Fraction_18121410_204.jpg

Cat No. GTX115154

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FUBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Far Upstream Element Binding Protein 1,Fbp,Fubp,Hdh V,Fubp1

Cellular Localization

Nucleus

Background

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq]

Database

DATA IMAGES
GTX115154_40401_IFA_18121410_420.jpg

GTX115154 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using FUBP1(GTX115154) antibody at 1:500 dilution.

GTX115154_40401_20150410_IFA_18121410_830.jpg

GTX115154 ICC/IF Image

FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (GTX115154) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX115154_40401_IHC_18121410_178.jpg

GTX115154 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using FUBP1(GTX115154) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115154_40401_20150410_IFA_2_18121410_965.jpg

GTX115154 ICC/IF Image

FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FUBP1 protein stained by FUBP1 antibody (GTX115154) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX115154_40401_20170420_WB_M_R_18121410_143.jpg

GTX115154 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX115154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115154_40401_20170420_WB_Fraction_18121410_204.jpg

GTX115154 WB Image

Orthogonal

Hela whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody (GTX115154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169