GeneTex
  • Country / Location Selection

United States (US)

FUS antibody [C1C3]

Anti-FUS antibody [C1C3] used in Western Blot (WB). GTX101810
Anti-FUS antibody [C1C3] used in Western Blot (WB). GTX101810
Anti-FUS antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101810
Anti-FUS antibody [C1C3] used in Western Blot (WB). GTX101810
Anti-FUS antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101810

Cat No. GTX101810

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FUS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FUS RNA binding protein , ALS6 , ETM4 , FUS1 , HNRNPP2 , POMP75 , TLS

Cellular Localization

Nucleus

Background

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-FUS antibody [C1C3] used in Western Blot (WB). GTX101810

GTX101810 WB Image

Wild-type (WT) and FUS knockout (KO) HEK293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [C1C3] (GTX101810) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUS antibody [C1C3] used in Western Blot (WB). GTX101810

GTX101810 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [C1C3] (GTX101810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUS antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101810

GTX101810 IHC-P Image

FUS antibody [C1C3] detects FUS protein at nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FUS antibody [C1C3] (GTX101810) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FUS antibody [C1C3] used in Western Blot (WB). GTX101810

GTX101810 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [C1C3] (GTX101810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUS antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101810

GTX101810 IHC-P Image

FUS antibody [C1C3] detects FUS protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
FUS antibody [C1C3] (GTX101810) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for FUS antibody [C1C3] (GTX101810). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FUS antibody [C1C3] (GTX101810). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf