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FUS antibody [HL2454]

Recombinant antibody KO/KD
Anti-FUS antibody [HL2454] used in Western Blot (WB). GTX638772
Anti-FUS antibody [HL2454] used in IHC (Paraffin sections) (IHC-P). GTX638772
Anti-FUS antibody [HL2454] used in IHC (Paraffin sections) (IHC-P). GTX638772
Anti-FUS antibody [HL2454] used in Western Blot (WB). GTX638772
Anti-FUS antibody [HL2454] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638772
Anti-FUS antibody [HL2454] used in Western Blot (WB). GTX638772

Cat. No. GTX638772

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2454

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FUS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human FUS.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FUS RNA binding protein , ALS6 , ETM4 , FUS1 , HNRNPP2 , POMP75 , TLS

Cellular Localization

Nucleus

Background

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-FUS antibody [HL2454] used in Western Blot (WB). GTX638772

GTX638772 WB Image

KO/KD

Wild-type (WT) and FUS knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (GTX638772) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUS antibody [HL2454] used in IHC (Paraffin sections) (IHC-P). GTX638772

GTX638772 IHC-P Image

FUS antibody [HL2454] detects FUS protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FUS stained by FUS antibody [HL2454] (GTX638772) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FUS antibody [HL2454] used in IHC (Paraffin sections) (IHC-P). GTX638772

GTX638772 IHC-P Image

FUS antibody [HL2454] detects FUS protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
FUS stained by FUS antibody [HL2454] (GTX638772) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FUS antibody [HL2454] used in Western Blot (WB). GTX638772

GTX638772 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (GTX638772) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FUS antibody [HL2454] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638772

GTX638772 ICC/IF Image

FUS antibody [HL2454] detects FUS protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% PFA.
Green: FUS stained by FUS antibody [HL2454] (GTX638772) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-FUS antibody [HL2454] used in Western Blot (WB). GTX638772

GTX638772 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (GTX638772) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations