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FUS antibody

KO/KD
Anti-FUS antibody used in Western Blot (WB). GTX55629
Anti-FUS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55629
Anti-FUS antibody used in Western Blot (WB). GTX55629
Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629
Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629
Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629
Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629
Anti-FUS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55629
Anti-FUS antibody used in Immunoprecipitation (IP). GTX55629

Cat. No. GTX55629

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all FUS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FUS RNA binding protein , ALS6 , ETM4 , FUS1 , HNRNPP2 , POMP75 , TLS

Cellular Localization

Nucleus

Background

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-FUS antibody used in Western Blot (WB). GTX55629

GTX55629 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX55629 FUS antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-FUS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55629

GTX55629 ICC/IF Image

ICC/IF analysis of C6 cells using GTX55629 FUS antibody.
Blue : DAPI
Dilution : 1:100

Anti-FUS antibody used in Western Blot (WB). GTX55629

GTX55629 WB Image

WB analysis of various sample lysates using GTX55629 FUS antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629

GTX55629 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX55629 FUS antibody.
Dilution : 1:100

Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629

GTX55629 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX55629 FUS antibody.
Dilution : 1:100

Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629

GTX55629 IHC-P Image

IHC-P analysis of rat brain tissue using GTX55629 FUS antibody.
Dilution : 1:100

Anti-FUS antibody used in IHC (Paraffin sections) (IHC-P). GTX55629

GTX55629 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX55629 FUS antibody.
Dilution : 1:100

Anti-FUS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55629

GTX55629 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX55629 FUS antibody.
Blue : DAPI
Dilution : 1:100

Anti-FUS antibody used in Immunoprecipitation (IP). GTX55629

GTX55629 IP Image

IP analysis of Jurkat cell lysate using GTX55629 FUS antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399