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FUZ antibody [N2C3]

Anti-FUZ antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX120464
Anti-FUZ antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120464
Anti-FUZ antibody [N2C3] used in Western Blot (WB). GTX120464
Anti-FUZ antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX120464

Cat. No. GTX120464

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all FUZ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FUZ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727701

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fuzzy planar cell polarity protein , CPLANE3 , FY , NTD

Cellular Localization

Cytoplasm , Cytoplasm , cytoskeleton

Background

May be involved in cytoskeleton function and cell polarity in epithelial cells.

Database

Research Area

DATA IMAGES

Anti-FUZ antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX120464

GTX120464 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using FUZ(GTX120464) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FUZ antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120464

GTX120464 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using FUZ(GTX120464) antibody at 1:500 dilution.

Anti-FUZ antibody [N2C3] used in Western Blot (WB). GTX120464

GTX120464 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX120464 diluted at 1:1000

Anti-FUZ antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX120464

GTX120464 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using FUZ(GTX120464) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169