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FXR1 antibody

Anti-FXR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113867
Anti-FXR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113867
Anti-FXR1 antibody used in Western Blot (WB). GTX113867
Anti-FXR1 antibody used in Western Blot (WB). GTX113867
Anti-FXR1 antibody used in Western Blot (WB). GTX113867
Anti-FXR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113867
Anti-FXR1 antibody used in Western Blot (WB). GTX113867
Anti-FXR1 antibody used in Western Blot (WB). GTX113867

Cat. No. GTX113867

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

HepG2 , Mouse liver

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FXR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036976

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FMR1 autosomal homolog 1 , FXR1P

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FXR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113867

GTX113867 IHC-P Image

Immunohistochemical analysis of paraffin-embedded sections of zebrafish kidney tube, using FXR1 antibody (GTX113867) at 1:300 dilution.

Anti-FXR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113867

GTX113867 ICC/IF Image

FXR1 antibody detects FXR1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FXR1 stained by FXR1 antibody (GTX113867) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-FXR1 antibody used in Western Blot (WB). GTX113867

GTX113867 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX113867 diluted at 1:1000

Anti-FXR1 antibody used in Western Blot (WB). GTX113867

GTX113867 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX113867 diluted at 1:1000

Anti-FXR1 antibody used in Western Blot (WB). GTX113867

GTX113867 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish brain
7.5% SDS PAGE
GTX113867 diluted at 1:1000

Anti-FXR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113867

GTX113867 IHC-P Image

FXR1 antibody detects FXR1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FXR1 stained by FXR1 antibody (GTX113867) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FXR1 antibody used in Western Blot (WB). GTX113867

GTX113867 WB Image

The data was published in the Front Vet Sci in 2020. PMID: 33195535

Anti-FXR1 antibody used in Western Blot (WB). GTX113867

GTX113867 WB Image

The data was published in the Front Vet Sci in 2020. PMID: 33195535

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169