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FYN antibody [2A10]

Anti-FYN antibody [2A10] used in Flow cytometry (FACS). GTX60460
Anti-FYN antibody [2A10] used in ELISA (ELISA). GTX60460
Anti-FYN antibody [2A10] used in Western Blot (WB). GTX60460
Anti-FYN antibody [2A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60460
Anti-FYN antibody [2A10] used in IHC (Paraffin sections) (IHC-P). GTX60460

Cat. No. GTX60460

Host

Mouse

Clonality

Monoclonal

Clone Name

2A10

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μl ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human FYN expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FYN proto-oncogene, Src family tyrosine kinase , SLK , SYN , p59-FYN

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FYN antibody [2A10] used in Flow cytometry (FACS). GTX60460

GTX60460 FACS Image

FACS analysis of HeLa cells using GTX60460 FYN antibody [2A10].
Green : FYN
Purple : negative control

Anti-FYN antibody [2A10] used in ELISA (ELISA). GTX60460

GTX60460 ELISA Image

ELISA analysis of antigen using GTX60460 FYN antibody [2A10].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-FYN antibody [2A10] used in Western Blot (WB). GTX60460

GTX60460 WB Image

WB analysis of human FYN (AA: 7-176) recombinant protein using GTX60460 FYN antibody [2A10].

Anti-FYN antibody [2A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60460

GTX60460 ICC/IF Image

ICC/IF analysis of U251 cells using GTX60460 FYN antibody [2A10].
Green : FYN
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-FYN antibody [2A10] used in IHC (Paraffin sections) (IHC-P). GTX60460

GTX60460 IHC-P Image

IHC-P analysis of breast cancer tissue (left) and brain tissue (right) using GTX60460 FYN antibody [2A10].

REFERENCE

There are currently no references for FYN antibody [2A10] (GTX60460). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FYN antibody [2A10] (GTX60460). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 409