APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89744-PEP).
Product Note
This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence VQCKDKEATKLTE, from the N Terminus of the protein sequence according to NP_002028.1; NP_694592.1; NP_694593.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
FYN proto-oncogene, Src family tyrosine kinase , SLK , SYN , p59-FYN
Cellular Localization
Cytoplasm , Nucleus , Cell membrane
Background
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89744 WB Image
WB analysis of A431 (A) and Jurkat lysate (B) using GTX89744 FYN antibody, N-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for FYN antibody, N-term (GTX89744). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FYN antibody, N-term (GTX89744). Be the first to share your experience with this product.