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FYN antibody [N1C2]

Anti-FYN antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109428
Anti-FYN antibody [N1C2] used in Western Blot (WB). GTX109428
Anti-FYN antibody [N1C2] used in Western Blot (WB). GTX109428

Cat. No. GTX109428

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FYN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Jurkat , Mouse brain , Rat brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729465

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FYN proto-oncogene, Src family tyrosine kinase , SLK , SYN , p59-FYN

Cellular Localization

Cell membrane

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FYN antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109428

GTX109428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FYN(GTX109428) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FYN antibody [N1C2] used in Western Blot (WB). GTX109428

GTX109428 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX109428 diluted at 1:1000

Anti-FYN antibody [N1C2] used in Western Blot (WB). GTX109428

GTX109428 WB Image

FYN antibody [N1C2] detects FYN protein by western blot analysis. Mouse and rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FYN antibody [N1C2] (GTX109428) diluted at 1:1000.

REFERENCE

Application Reference

Lee JH et al. EBioMedicine 2018; (Epub) HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169