GeneTex
United States (US)

FYN (phospho Tyr530) antibody

GTX32185_20151013_IHC-P_18121410_641.jpg
GTX32185_20151013_WB_18121410_240.jpg

Cat No. GTX32185

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

61kDa kDa. ( Note )

Specificity/Sensitivity

Recognizes endogenous levels of FYN (pY530) protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FYN. The exact sequence is proprietary.

Purification

The antibody was purified by immunogen affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FYN proto-oncogene, Src family tyrosine kinase Antibody , SLK Antibody , p59-FYN Antibody , FYN Antibody , SYN Antibody

Cellular Localization

Cell membrane

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX32185_20151013_IHC-P_18121410_641.jpg

GTX32185 IHC-P Image

Immunohistochemical analysis of FYN (pY530) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

GTX32185_20151013_WB_18121410_240.jpg

GTX32185 WB Image

Western blot analysis of FYN (pY530) expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.