FYN (phospho Tyr530) antibody

Cat No. GTX32185

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

61kDa kDa. ( Note )

Specificity/Sensitivity

Recognizes endogenous levels of FYN (pY530) protein.

PROPERTIES

Form

Liquid

Buffer

Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FYN. The exact sequence is proprietary.

Purification

The antibody was purified by immunogen affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FYN proto-oncogene, Src family tyrosine kinase Antibody , SLK Antibody , p59-FYN Antibody , FYN Antibody , SYN Antibody

Cellular Localization

Cell membrane

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

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Database

Gene ID:2534
UniProt:P06241

Research Area

DATA IMAGES

GTX32185 IHC-P Image

Immunohistochemical analysis of FYN (pY530) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.