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FZR1 antibody

Orthogonal
Anti-FZR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111200
Anti-FZR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111200
Anti-FZR1 antibody used in Western Blot (WB). GTX111200
Anti-FZR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111200
Anti-FZR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111200

Cat. No. GTX111200

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FZR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , A431 nuclear extract

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FZR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173217

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fizzy and cell division cycle 20 related 1 , CDC20C , CDH1 , FZR , FZR2 , HCDH , HCDH1

Cellular Localization

Isoform 2: Nucleus , Isoform 3: Cytoplasm

Background

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Database

Research Area

DATA IMAGES

Anti-FZR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111200

GTX111200 IHC-P Image

FZR1 antibody detects FZR1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FZR1 antibody (GTX111200) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FZR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111200

GTX111200 IHC-P Image

FZR1 antibody detects FZR1 protein at nucleus in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
FZR1 antibody (GTX111200) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FZR1 antibody used in Western Blot (WB). GTX111200

GTX111200 WB Image

Orthogonal

A431 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FZR1/CDH1 antibody (GTX111200) diluted at 1:1000.

Anti-FZR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111200

GTX111200 IHC-P Image

FZR1 antibody detects FZR1 protein at nucleus in mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
FZR1 antibody (GTX111200) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FZR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111200

GTX111200 ICC/IF Image

FZR1 antibody detects FZR1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FZR1 protein stained by FZR1 antibody (GTX111200) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

REFERENCE

Application Reference

Wang T et al. Cell Rep 2019; 29 (7) : 2078-2091.e5 CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation.
Application : WB
Reactivity : Human
Yu-Ting Peng et al. Toxicology Reports 2015; 2 : 322-332 Upregulation of cyclin-dependent kinase inhibitors CDKN1B and CDKN1C in hepatocellular carcinoma-derived cells via goniothalamin-mediated protein stabilization and epigenetic modifications
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169