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Factor B antibody [N2C2], Internal

Anti-Factor B antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103570
Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570
Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570

Cat No. GTX103570

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Raji , K562 , Rat liver

Predict Reactivity

Mouse, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Factor B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

complement factor B , AHUS4 , ARMD14 , BF , BFD , CFAB , CFBD , FB , FBI12 , GBG , H2-Bf , PBF2

Cellular Localization

Secreted

Background

This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Factor B antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103570

GTX103570 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Factor B(GTX103570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570

GTX103570 WB Image

Human plasma (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal (GTX103570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Factor B antibody [N2C2], Internal used in Western Blot (WB). GTX103570

GTX103570 WB Image

Rat plasma (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal (GTX103570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf