GeneTex

  • Country / Location Selection

United States (US)

Factor B (cleaved Lys260) antibody

Anti-Factor B (cleaved Lys260) antibody used in Western Blot (WB). GTX86947

Cat. No. GTX86947

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all Factor B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Product Note

CFAB Bb (cleaved Lys260) antibody detects endogenous levels of fragment of activated CFAB Bb resulting from cleavage adjacent to Lys260.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CFAB Bb (241-290).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10723596

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AHUS4 , ARMD14 , BF , BFD , CFAB , CFB , CFBD , FB , FBI12 , GBG , H2Bf , PBF2 , complement factor B , Factor B , Complement Factor B

Cellular Localization

Secreted

Background

This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Factor B (cleaved Lys260) antibody used in Western Blot (WB). GTX86947

GTX86947 WB Image

WB analysis of K562 cells treated with etoposide 25uM (1h) lysate using GTX86947 CFAB Bb (cleaved Lys260) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Belcher JD et al. Transl Res 2022; 249 : 44938 MASP-2 and MASP-3 inhibitors block complement activation, inflammation, and microvascular stasis in a murine model of vaso-occlusion in sickle cell disease.
Vercellotti GM et al. Am J Hematol 2019; 94 (3) : 327-337 Critical role of C5a in sickle cell disease.
Submit a Reference

REVIEW

There are currently no reviews for Factor B (cleaved Lys260) antibody (GTX86947). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399