GeneTex
United States (US)

Factor H antibody [N3C1], Internal

GTX100305_39435_20160204_WB_H_plasma_19011506_477.jpg
GTX100305_WB_19011506_422.jpg
GTX100305_39435_IHC_19011506_620.jpg

Cat No. GTX100305

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

Molt-4 , human plasma
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Factor H. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AHUS1 antibody, AMBP1 antibody, ARMD4 antibody, ARMS1 antibody, CFHL3 antibody, FH antibody, FHL1 antibody, HF antibody, HF1 antibody, HF2 antibody, HUS antibody, MGC88246 antibody, CFH antibody, beta-1H antibody, H factor 1 (complement) antibody, "complement factor H, isoform b antibody", age-related maculopathy susceptibility 1 antibody, beta-1-H-globulin antibody, adrenomedullin binding protein antibody, H factor 2 (complement) antibody, factor H-like 1 antibody, complement factor H antibody, factor H antibody

Cellular Localization

Secreted

Background

This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100305_39435_20160204_WB_H_plasma_19011506_477.jpg

GTX100305 WB Image

Human tissue extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Factor H antibody [N3C1], Internal (GTX100305) diluted at 1:500.

GTX100305_WB_19011506_422.jpg

GTX100305 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
5% SDS PAGE
GTX100305 diluted at 1:5000

GTX100305_39435_IHC_19011506_620.jpg

GTX100305 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Factor H(GTX100305) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169