APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Recognises the heavy chain of Factor IX
PROPERTIES
Form
Liquid
Buffer
50mM Sodium Phosphate, 100mM NaCl, 1mM EDTA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length native protein (purified) (Human).
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_11162678
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
F9,F9 p22,FIX,HEMB,P19,PTC,THPH8,coagulation factor IX,Factor IX
Cellular Localization
Secreted
Background
This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
![Anti-Factor IX antibody [13F42-F6] used in Western Blot (WB). GTX79803](/templates/default/js/lightbox/images/loading.gif "Anti-Factor IX antibody [13F42-F6] used in Western Blot (WB). GTX79803")
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GTX79803 WB Image
WB analysis of various sample lysates using GTX79803 Factor IX antibody [13F42-F6].
Lane 1 : Human Factor IX (0.25 μg) Non-reduced
Lane 2 : Human Factor IXa (0.25 μg) Non-reduced
Lane 3 : Human Factor IX (0.25 μg) Reduced
Lane 4 : Human Factor IXa (0.25 μg) Reduced
Lane 5 : Molecular Weight Markers
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REFERENCE
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REVIEW
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![Anti-Factor IX antibody [13F42-F6] used in Western Blot (WB). GTX79803](/upload/website/prouct_img/small/GTX79803/GTX79803-1_w_23061322_164.webp "Anti-Factor IX antibody [13F42-F6] used in Western Blot (WB). GTX79803")
![Anti-Factor IX antibody [13F42-F6] used in Western Blot (WB). GTX79803](/upload/website/prouct_img/normal/GTX79803/GTX79803-1_w_23061322_164.webp "Anti-Factor IX antibody [13F42-F6] used in Western Blot (WB). GTX79803")