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Factor IX antibody [N3C3]

Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689
Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689
Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689
Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689
Anti-Factor IX antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113689

Cat. No. GTX113689

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Factor IX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Factor IX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036886

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor IX , F9 p22 , FIX , HEMB , P19 , PTC , THPH8

Cellular Localization

Secreted

Background

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689

GTX113689 IHC-P Image

Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Factor IX antibody [N3C3] (GTX113689) diluted at 1:250.
Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689

GTX113689 IHC-P Image

Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Factor IX antibody [N3C3] (GTX113689) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689

GTX113689 IHC-P Image

Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Factor IX antibody [N3C3] (GTX113689) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Factor IX antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113689

GTX113689 IHC-P Image

Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Factor IX stained by Factor IX antibody [N3C3] (GTX113689) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Factor IX antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113689

GTX113689 ICC/IF Image

Factor IX antibody [N3C3] detects secreted Factor IX protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Factor IX protein stained by Factor IX antibody [N3C3] (GTX113689) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Sato R et al. J Vet Med Sci 2022; 84 (6) : 804-808 Development of a sandwich enzyme-linked immunosorbent assay (ELISA) to quantify γ-glutamyl-carboxylated clotting factor IX and assess redox susceptibility of anticoagulant chemicals.
Application : WB, ELISA
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169