APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Factor IX. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036886
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
coagulation factor IX , F9 p22 , FIX , HEMB , P19 , PTC , THPH8
Cellular Localization
Secreted
Background
This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113689 IHC-P Image
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Factor IX antibody [N3C3] (GTX113689) diluted at 1:250.
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GTX113689 IHC-P Image
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Factor IX antibody [N3C3] (GTX113689) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113689 IHC-P Image
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Factor IX antibody [N3C3] (GTX113689) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113689 IHC-P Image
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Factor IX stained by Factor IX antibody [N3C3] (GTX113689) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113689 ICC/IF Image
Factor IX antibody [N3C3] detects secreted Factor IX protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX protein stained by Factor IX antibody [N3C3] (GTX113689) diluted at 1:500. Blue: Hoechst 33342 staining.
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REFERENCE
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