GeneTex
United States (US)

Factor VII antibody [N3C3]

GTX101238_WB_M_19011506_959.jpg
GTX101238_39883_20160218_WB_H_plasma_19011506_218.jpg
GTX101238_IHC_19011506_626.jpg

Cat No. GTX101238

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

human plasma , NIH-3T3

Specificity/Sensitivity

Upon activation of the factor VII, proteolytic cleavage of the peptide bond between Arg(152) and Ile(153) converts Factor VII (FVII) to an activated two-chain form (FVIIa). A heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated. Since the immunogen sequence locates within the heavy chain, it should recognize both Factor VII and Factor VIIa.

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Factor VII. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Coagulation Factor Vii,Spca,F7

Cellular Localization

Secreted

Background

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101238_WB_M_19011506_959.jpg

GTX101238 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
10% SDS PAGE
GTX101238 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101238_39883_20160218_WB_H_plasma_19011506_218.jpg

GTX101238 WB Image

Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Factor VII antibody [N3C3] (GTX101238) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101238_IHC_19011506_626.jpg

GTX101238 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal gastric epithelium (gland) , using F7(GTX101238) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf