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Factor VII antibody [N3C3]

Anti-Factor VII antibody [N3C3] used in Western Blot (WB). GTX101238
Anti-Factor VII antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101238
Anti-Factor VII antibody [N3C3] used in Western Blot (WB). GTX101238

Cat. No. GTX101238

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Factor VII products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

NIH-3T3

Product Note

Upon activation of the factor VII, proteolytic cleavage of the peptide bond between Arg(152) and Ile(153) converts Factor VII (FVII) to an activated two-chain form (FVIIa). A heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated. Since the immunogen sequence locates within the heavy chain, it should recognize both Factor VII and Factor VIIa.

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Factor VII. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950245

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor VII , SPCA

Cellular Localization

Secreted

Background

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Factor VII antibody [N3C3] used in Western Blot (WB). GTX101238

GTX101238 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
10% SDS PAGE
GTX101238 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Factor VII antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101238

GTX101238 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal gastric epithelium (gland) , using F7(GTX101238) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Factor VII antibody [N3C3] used in Western Blot (WB). GTX101238

GTX101238 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Factor VII antibody [N3C3] (GTX101238) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yang L et al. Oncotarget 2018; 9 (5) : 5614-5626 Anticoagulants inhibit proteolytic clearance of plasma amyloid beta.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169