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Factor X antibody

Orthogonal
Anti-Factor X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109830
Anti-Factor X antibody used in Western Blot (WB). GTX109830
Anti-Factor X antibody used in IHC (Paraffin sections) (IHC-P). GTX109830
Anti-Factor X antibody used in Western Blot (WB). GTX109830
Anti-Factor X antibody used in Western Blot (WB). GTX109830

Cat. No. GTX109830

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Factor X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

human F10-transfected 293T cells , rat liver

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Factor X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950239

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor X , FX , FXA

Cellular Localization

Secreted

Background

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Factor X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109830

GTX109830 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Factor X(GTX109830) antibody at 1:200 dilution.

Anti-Factor X antibody used in Western Blot (WB). GTX109830

GTX109830 WB Image

Factor X antibody detects F10 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells
10 % SDS-PAGE
Factor X antibody (GTX109830) dilution: 1:5000

Anti-Factor X antibody used in IHC (Paraffin sections) (IHC-P). GTX109830

GTX109830 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Factor X(GTX109830) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Factor X antibody used in Western Blot (WB). GTX109830

GTX109830 WB Image

Orthogonal

HepG2 conditioned medium (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody (GTX109830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Factor X antibody used in Western Blot (WB). GTX109830

GTX109830 WB Image

Rat tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody (GTX109830) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169